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Transgene Detail
Symbol: Tg(APPSwe,tauP301L)1Lfa
Name: transgene insertion 1, Frank M LaFerla
MGI ID: MGI:2672831
Synonyms: Tg(APP*Swe,MAPT*P301L)1Lfa
Transgene: Tg(APPSwe,tauP301L)1Lfa  Location: Chr2:87692807-87692810 bp  Genetic Position: Chr2, Syntenic
Alliance: Tg(APPSwe,tauP301L)1Lfa page
Strain of Origin:  Not Specified
Transgene Type:    Transgenic (Humanized sequence, Inserted expressed sequence)
Mutations:    Insertion, Intergenic deletion
Tg(APPSwe,tauP301L)1Lfa expresses 2 genes
Mutation detailsTo develop a model of Alzheimer's Disease, mice harboring mutant human APP (Swedish double mutation; K670N, M671L) and MAPT (P301L) as well as Psentm1Mpm were generated by microinjection of the APP and MAPT transgenic constructs into single cell embryos harvested from mice homozygous for Psen1tm1Mpm. Southern blot analysis indicated that both transgenic constructs integrated into the same site. Western blot analysis showed APP and MAPT levels to be ~4 fold higher in hemizygous mice and ~6 (APP) to ~7 (Mapt) fold higher homozygous mice, relative to non transgenic mice. Amyloid-Beta peptide (both 40 and 42) was detected in transgenic mice, with greater levels in homozygous mice than in hemizygous mice. Expression was confined to the CNS. Highest steady state levels of proteins were detected in Alzheimer's Disease related regions including the hippocampus and cerebral cortex. Transgenic protein was not detected in the cerebellum. The transgene inserted on Chr 2 causing a 3 bp deletion. (J:84847)
View phenotypes and curated references for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 3 strains available      Cell Lines: 3 lines available
Original:  J:84847 Oddo S, et al., Triple-transgenic model of Alzheimer's disease with plaques and tangles: intracellular Abeta and synaptic dysfunction. Neuron. 2003 Jul 31;39(3):409-21
All:  504 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB)), Gene Ontology (GO)
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MGI 6.22
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