Ses2^129/Sv
QTL Variant Detail

Summary

• QTL variant: Ses2^129/Sv
• Name: salmonella enteritidis susceptibility 2; 129/Sv
• MGI ID: MGI:2668909
• QTL: Ses2 Location: Chr7:84290094-84290240 bp Genetic Position: Chr7, cM position of peak correlated region/allele: 48.36 cM
• QTL Note: genome coordinates based on the marker associated with the peak LOD score

Variant origin

• Strain of Specimen: 129/Sv

Variant description

• Allele Type: QTL
• Mutation: Undefined
• This allele confers susceptibility to Salmonella enteritidis infection compared to C57BL/6J.
• Inheritance: Dominant

Notes

Mapping and Phenotype information for this QTL, its variants and associated markers

J:81412

Genome scan using 80 polymorphic markers at an average spacing of 20 cM was performed on a population of (C57BL/6J x 129/Sv)F2 animals to identify QTLs associated with resistance to Salmonella enteritidis infection. Parental strain C57BL/6J is resistant to S.enteritidis and clears an infection by 42 days post-innoculation while parental strain 129/Sv is susceptible and cannot clear an infection after 42 days. Three major QTLs named Ses1, Ses2, and Ses3 were mapped to chromosomes 1, 7, and 15, respectively. Together these QTLs explain 32% of the phenotypic variance.

Ses1 maps to 48.7 cM on mouse Chromosome 1 with a peak LOD score = 9.9 at D1Mcg5. Ses1 spans 23.1 cM and explains 14% of the phenotypic variance. Ses2 maps to 33.3 cM on mouse Chromosome 7 with a peak LOD score = 4 at D7Mit62. Ses2 spans 29.1 cM and explains 6% of the genetic variance. Ses3 maps to 38.8 cM on mouse Chromosome 15 with a peak LOD score = 3.2 at D15Mit29. Ses3 spans 20.6 cM and explains 4% of the phenotypic variance.

C57BL/6J-derived alleles confer recessively inherited resistance to S. enteritidis infection at all 3 QTLs. Linkage to S.enteritidis resistance shows greater significance in female animals at all loci.

Slc11a1 (Nramp1) contains a functional polymorphism (Asp169)in C57BL/6J and is a possible candidate gene for Ses1. The C57BL/6J allele is a null allele and is linked to susceptibility to Salmonella typhimurium infection. 129/Sv animals carrying the Asp169 allele exhibit lower S. enteritidis CFU counts compared to129/Sv animals. Thus, Slc11a1 is a viable candidate gene and may play a role in bacterial clearance.

References

• Original: J:81412 Caron J, et al., Identification of genetic loci controlling bacterial clearance in experimental Salmonella enteritidis infection: an unexpected role of Nramp1 (Slc11a1) in the persistence of infection in mice. Genes Immun. 2002 Jun;3(4):196-204
• All: 1 reference(s)