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Mt2tm1Rch
Targeted Allele Detail
Nomenclature
Symbol: Mt2tm1Rch
Name: metallothionein 2; targeted mutation 1, Anna E Michalska
MGI ID: MGI:2668455
Synonyms: MT-II -, MT-null
Gene: Mt2  Location: Chr8:94172618-94173567 bp, + strand  Genetic Position: Chr8, 46.32 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:14416
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsBoth metallothionein I and II were disrupted using the same targeting construct. The second exon of metallothionein II had an insertion of the pMC1NeoPA selection marker at a position 30bp downstream of the start codon. Northern blot analysis of liver mRNA from homozygotes in which the gene was induced by intraperitoneal injection of copper demonstrated that transcription was prevented. Further, no protein product was detected in the livers of these animals. (J:14416)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Mt2 Mutation:  5 strains or lines available
References
Original:  J:14416 Michalska AE, et al., Targeting and germ-line transmission of a null mutation at the metallothionein I and II loci in mouse. Proc Natl Acad Sci U S A. 1993 Sep 1;90(17):8088-92
All:  23 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
09/20/2016
MGI 6.05
The Jackson Laboratory