Tg(CD2-CD4)313Flv
Transgene Detail

Summary

• Symbol: Tg(CD2-CD4)313Flv
• Name: transgene insertion 313, Richard A Flavell
• MGI ID: MGI:2665755
• Synonyms: TG huCD4
• Transgene: Tg(CD2-CD4)313Flv Location: unknown
• Alliance: Tg(CD2-CD4)313Flv page

Transgene origin

• Strain of Origin: (CBA/Ca x C57BL/10)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(CD2-CD4)313Flv expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CD4 (920)

  Cd4	(MGI:88335)

• Mutation details: Sequence encoding human CD4 was inserted into a CD2 minigene cassette that contains 4.8 Kb of human CD2 promoter sequence and 5.5 Kb of downstream flanking sequence. Eight transgenic lines were established and line 313, which had 15 to 20 copies of the inserted transgene, was selected for further breeding and study. Human CD4 is expressed in >95% of transgenic T cells as observed by FACS analysis. (J:131345)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

The construction of this human CD4 transgenic line (313) is briefly described in J:77270. Details are said to be described elsewhere by Kaufman-Paterson (manuscript submitted for publication) but I have not been able to locate this reference -IL (7/17/03) I came across the paper describing the transgene, and chanced the molecular reference and the molecular note. TFM 3-14-08

References

• Original: J:77270 Law YM, et al., Human CD4 restores normal T cell development and function in mice deficient in murine CD4. J Exp Med. 1994 Apr 1;179(4):1233-42
• All: 7 reference(s)