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Targeted Allele Detail
Symbol: Cd28tm1Pjl
Name: CD28 antigen; targeted mutation 1, Philip J Lucas
MGI ID: MGI:2664380
Synonyms: CD28-
Gene: Cd28  Location: Chr1:60746388-60773359 bp, + strand  Genetic Position: Chr1, 30.52 cM, cytoband C1-C3
Germline Transmission:  Earliest citation of germline transmission: J:25536
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThe gene was disrupted by replacement of a 0.5 kb region containing most of exon 2 with a neomycin resistance cassette. Exon 2 encodes the Ig-like region, an alternative splice site, a putative ligand-binding domain, and represents more than 70% of the extracellular domain. Absence of gene expression was verified by flow cytometry of CD4+ T cells from homozygous mutant animals. (J:25536)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Cd28 Mutation:  29 strains or lines available
Original:  J:25536 Lucas PJ, et al., Naive CD28-deficient T cells can initiate but not sustain an in vitro antigen-specific immune response. J Immunol. 1995 Jun 1;154(11):5757-68
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory