About   Help   FAQ
Targeted Allele Detail
Symbol: Gusbtm4Sly
Name: glucuronidase, beta; targeted mutation 4, William S Sly
MGI ID: MGI:2661042
Synonyms: Gustm(hE540A.mE536A)Sly
Gene: Gusb  Location: Chr5:129989011-130003049 bp, - strand  Genetic Position: Chr5, 68.32 cM
Germline Transmission:  Earliest citation of germline transmission: J:83169
Parent Cell Line:  RW-4 (ES Cell)
Strain of Origin:  129X1/SvJ
Allele Type:    Targeted
Mutations:    Insertion, Nucleotide substitutions
Mutation detailsMolecular techniques were employed to generate an allele containing E536A in exon 10 and a cDNA sequence encoding an inactive form of human GUS in intron 9. The human cDNA contained the E540A missense mutation. Inactive forms of both human and murine protein were produced, resulting in mutant mice that possess immunologic tolerance to both human and murine wild-type enzymes. (J:83169)
View phenotypes for all genotypes (concatenated display).
Disease models
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Gusb Mutation:  29 strains or lines available
Original:  J:83169 Tomatsu S, et al., Production of MPS VII mouse (Gus(tm(hE540A.mE536A)Sly)) doubly tolerant to human and mouse beta-glucuronidase. Hum Mol Genet. 2003 May 1;12(9):961-73
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
Citing These Resources
Funding Information
Warranty Disclaimer & Copyright Notice
Send questions and comments to User Support.
last database update
MGI 6.03
The Jackson Laboratory