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Targeted Allele Detail
Symbol: Wt1tm1Hst
Name: Wilms tumor 1 homolog; targeted mutation 1, Nicholas D Hastie
MGI ID: MGI:2654351
Gene: Wt1  Location: Chr2:105126529-105173616 bp, + strand  Genetic Position: Chr2, 55.06 cM, cytoband E
Germline Transmission:  Earliest citation of germline transmission: J:82515
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Modified isoform(s), Null/knockout)
Mutations:    Insertion, Single point mutation
Mutation detailsA nonsense mutation was introduced into exon 1, 12 codons downstream of the CUG start codon and upstream of the AUG start codon. A floxed hygro cassette was excised in vitro, leaving a single loxP site in a nonconserved region within in intron 1. Western blot analysis of homozygous mutant fetal kidney and testis tissue showed that while the CUG-initiated isoforms were undetected, normal levels of AUG-initiated isoforms were present. (J:82515)
View phenotypes and curated references for all genotypes (concatenated display).
In Mice Carrying this Mutation: 3 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Wt1 Mutation:  21 strains or lines available
Original:  J:82515 Miles CG, et al., Mice lacking the 68-amino-acid, mammal-specific N-terminal extension of WT1 develop normally and are fertile. Mol Cell Biol. 2003 Apr;23(7):2608-13
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.12
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