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Krt14tm2Der
Targeted Allele Detail
Nomenclature
Symbol: Krt14tm2Der
Name: keratin 14; targeted mutation 2, Dennis R Roop
MGI ID: MGI:2653130
Synonyms: mtK14loxP
Gene: Krt14  Location: Chr11:100203162-100207548 bp, - strand  Genetic Position: Chr11, 63.43 cM, cytoband D
Blistering on the left front leg and abdomen of Krt14tm2Der/Krt14+ pups

Show the 2 phenotype image(s) involving this allele.

Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:67320
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  129X1/SvJ
Mutation
description
Allele Type:    Targeted
Mutation:    Insertion
 
Mutation detailsA C to T point mutation at codon 131 was introduced to the gene and a floxed PGK-neo cassette was inserted into the downstream exon via homologous recombination. The point mutation results in an arginine to cysteine substitution. Transient expression of cre recombinase in correctly targeted ES cells deleted the floxed PGK-neo cassette leaving a single loxP site in its place. The targeted allele was confirmed by Southern blot and sequence analysis. (J:67320)
Phenotypes
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Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Krt14 Mutation:  1 strain or line available
References
Original:  J:67320 Cao T, et al., An Inducible Mouse Model for Epidermolysis Bullosa Simplex. Implications for gene therapy. J Cell Biol. 2001 Feb 5;152(3):651-6
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
06/23/2015
MGI 5.22
The Jackson Laboratory