Krt14^tm1Der
Targeted Allele Detail

Summary

• Symbol: Krt14^tm1Der
• Name: keratin 14; targeted mutation 1, Dennis R Roop
• MGI ID: MGI:2653126
• Synonyms: mtK14^neo, mut^neo
• Gene: Krt14 Location: Chr11:100093988-100098336 bp, - strand Genetic Position: Chr11, 63.43 cM, cytoband D
• Alliance: Krt14^tm1Der page

Severe blisters on the front legs, paws, and chest of Krt14^tm1Der/Krt14^tm1Der pups

Show the 1 phenotype image(s) involving this allele.

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:67320
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: 129X1/SvJ

Mutation description

• Allele Type: Targeted (Not Specified)
• Mutations: Insertion, Single point mutation
• Mutation details: A C to T point mutation at codon 131 was introduced to the gene and a floxed PGK-neo cassette was inserted into the downstream exon via homologous recombination. The point mutation results in an arginine to cysteine substitution. The targeted allele was confirmed by Southern blot and sequence analysis. (J:67320)

Disease models

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Krt14 Mutation: 36 strains or lines available

References

• Original: J:67320 Cao T, et al., An Inducible Mouse Model for Epidermolysis Bullosa Simplex. Implications for gene therapy. J Cell Biol. 2001 Feb 5;152(3):651-6
• All: 2 reference(s)