Ndn^tm1Mus
Targeted Allele Detail

Summary

• Symbol: Ndn^tm1Mus
• Name: necdin, MAGE family member; targeted mutation 1, Francoise Muscatelli
• MGI ID: MGI:2653059
• Gene: Ndn Location: Chr7:61998025-61999676 bp, + strand Genetic Position: Chr7, 34.36 cM, cytoband C
• Alliance: Ndn^tm1Mus page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:66557
• Parent Cell Line: CK35 (ES Cell)
• Strain of Origin: 129S2/SvPas

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The 5' two-thirds of the endogenous coding region was replaced with a floxed neo cassette inserted by homologous recombination. Normal transcript was undetected by Northern blot and RT-PCR analyses of both homozygous mutant mice and heterozygous mice carrying a paternal mutant allele. An absence of protein was verified in these mice by Western blot analysis of brain tissue. (J:66557)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Ndn Mutation: 18 strains or lines available

References

• Original: J:66557 Muscatelli F, et al., Disruption of the mouse necdin gene results in hypothalamic and behavioral alterations reminiscent of the human prader-willi syndrome. Hum Mol Genet. 2000 Dec 12;9(20):3101-10
• All: 4 reference(s)