Hbb-bh1^tm1Fier
Targeted Allele Detail

Summary

• Symbol: Hbb-bh1^tm1Fier
• Name: hemoglobin Z, beta-like embryonic chain; targeted mutation 1, Steven Fiering
• MGI ID: MGI:2652935
• Synonyms: deltabetah1
• Gene: Hbb-bh1 Location: Chr7:103490845-103492369 bp, - strand Genetic Position: Chr7, 54.85 cM
• Alliance: Hbb-bh1^tm1Fier page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:82296
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 1 and exon 2 with a floxed PGK-neo cassette via homologous recombination. The targeted mutation results in deletion of a 1.1 kb of DNA including the promoter region. Absence of gene expression in homozygous mutant animals was confirmed by RT-PCR analysis of embryonic yolk sac RNA. (J:82296)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hbb-bh1 Mutation: 8 strains or lines available

References

• Original: J:82296 Hu X, et al., Promoters of the murine embryonic beta-like globin genes Ey and betah1 do not compete for interaction with the beta-globin locus control region. Proc Natl Acad Sci U S A. 2003 Feb 4;100(3):1111-5
• All: 3 reference(s)