Tg(Prnp)F35Cwe
Transgene Detail

Summary

• Symbol: Tg(Prnp)F35Cwe
• Name: transgene insertion F35, Charles Weissmann
• MGI ID: MGI:2652453
• Synonyms: delta PrP, F35, PrPdelta32-134, PrP^deltaF, Tg(F35)
• Transgene: Tg(Prnp)F35Cwe Location: unknown
• Alliance: Tg(Prnp)F35Cwe page

Transgene origin

• Strain of Origin: B6;129S7-Prnp^tm1Cwe/CweCnrm

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Prnp)F35Cwe expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Prnp (MGI:97769)

• Mutation details: A transgenic construct was engineered to contain murine Prnp lacking sequence encoding amino acids 32 through 134. Sequence, including that encoding the amino-terminal signal sequence, necassary for proper transport to the endoplasmic reticulum and proper processing was left intact. Transgenic expression was verified by Western blot analysis. Only after treatment with PNGase did the transgenic protein migrate a distance corresponding to the deletion, indicating in vivo glycosylation. A carboxy terminal cleavage product was also detected. (J:47034)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:47034 Shmerling D, et al., Expression of amino-terminally truncated PrP in the mouse leading to ataxia and specific cerebellar lesions. Cell. 1998 Apr 17;93(2):203-14
• All: 11 reference(s)