Trp53^tm2Brd
Targeted Allele Detail

Summary

• Symbol: Trp53^tm2Brd
• Name: transformation related protein 53; targeted mutation 2, Allan Bradley
• MGI ID: MGI:2450287
• Synonyms: Trp53^{In(11Trp53;11Wnt3)8Brd}, Trp53^tyr, Tyrp53^Brdm2
• Gene: Trp53 Location: Chr11:69471185-69482699 bp, + strand Genetic Position: Chr11, 42.83 cM, cytoband B2-C
• Alliance: Trp53^tm2Brd page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:56548
• Parent Cell Line: AB2.2 (ES Cell)
• Strain of Origin: 129S7/SvEvBrd-Hprt1^b-m2

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutations: Insertion, Intragenic deletion
• Trp53^tm2Brd involves 1 genes/genome features (Trp53) View all
• Mutation details: A 0.6-kb genomic DNA fragment containing exon 2, including the translation initiation codon, exon 3, and part of exon 4 was replaced with a cassette containing a puromycin resistance gene, a loxP site oriented distally (away from the centromere), the 3' half of an Hprt minigene, and a tyrosinase (Tyr) minigene in an ES cell line already harboring Wnt3^tm2Brd. The cassette integrated into the same Chromosome 16 homolog, in cis with Wnt3^tm2Brd. (J:56548, J:80303)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Trp53 Mutation: 232 strains or lines available

Notes

Oppositely-oriented loxP sites in Trp53^tm2Brd and Wnt3^tm2Brd, in cis, served as the proximal and distal breakpoints, respectively, of a 24-cM Cre recombinase-mediated inversion, In(11Trp53;11Wnt3)8Brd. (J:56548) Passage of the two targeted mutations through the germline without exposure to Cre recombinase resulted in their segregation to separate Chr 16 homologs and thus to their independent inheritance. (J:80303 )

References

• Original: J:56548 Zheng B, et al., Engineering a mouse balancer chromosome. Nat Genet. 1999 Aug;22(4):375-8
• All: 5 reference(s)