Egfr^tm1Arge
Targeted Allele Detail

Summary

• Symbol: Egfr^tm1Arge
• Name: epidermal growth factor receptor; targeted mutation 1, Argiris Efstratiadis
• MGI ID: MGI:2450245
• Synonyms: Egfr^lox
• Gene: Egfr Location: Chr11:16702203-16868158 bp, + strand Genetic Position: Chr11, 9.41 cM, cytoband A1-A4
• Alliance: Egfr^tm1Arge page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:60711
• Parent Cell Line: Not Specified (ES Cell)
• Strain of Origin: Not Specified

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: LoxP sites were inserted to flank exons 9 and 10 of the gene. A LacZ reporter with a nuclear localization signal was inserted upstream of the first LoxP site and exon 9. After exon 10, a STOP sequence and a neo selection cassette were inserted preceding the LoxP site, and a CMV minimal promoter was inserter after the loxP site. These insertions were reported to interfere with normal expression of the gene and resulted in a null allele. Upon Cre-mediated excision, exons 9 and 10, the STOP sequence and the selection cassette are removed, juxtaposing the CMV promoter and the nls-LacZ reporter so that reporter expression provides a readout of excision in the tissue. (J:60711)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Egfr Mutation: 85 strains or lines available

References

• Original: J:60711 Dietrich P, et al., Conditional mutagenesis in mice with heat shock promoter-driven cre transgenes. Mamm Genome. 2000 Mar;11(3):196-205
• All: 1 reference(s)