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Egfrtm1Arge
Targeted Allele Detail
Nomenclature
Symbol: Egfrtm1Arge
Name: epidermal growth factor receptor; targeted mutation 1, Argiris Efstratiadis
MGI ID: MGI:2450245
Synonyms: Egfrlox
Gene: Egfr  Location: Chr11:16752203-16913907 bp, + strand  Genetic Position: Chr11, 9.41 cM, cytoband A1-A4
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:60711
Parent Cell Line:  Not Specified (ES Cell)
Strain of Origin:  Not Specified
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Insertion
 
Mutation detailsLoxP sites were inserted to flank exons 9 and 10 of the gene. A LacZ reporter with a nuclear localization signal was inserted upstream of the first LoxP site and exon 9. After exon 10, a STOP sequence and a neo selection cassette were inserted preceding the LoxP site, and a CMV minimal promoter was inserter after the loxP site. These insertions were reported to interfere with normal expression of the gene and resulted in a null allele. Upon Cre-mediated excision, exons 9 and 10, the STOP sequence and the selection cassette are removed, juxtaposing the CMV promoter and the nls-LacZ reporter so that reporter expression provides a readout of excision in the tissue. (J:60711)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Egfr Mutation:  14 strains or lines available
References
Original:  J:60711 Dietrich P, et al., Conditional mutagenesis in mice with heat shock promoter-driven cre transgenes. Mamm Genome. 2000 Mar;11(3):196-205
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
12/03/2019
MGI 6.14
The Jackson Laboratory