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Targeted Allele Detail
Symbol: Btktm1Gsv
Name: Bruton agammaglobulinemia tyrosine kinase; targeted mutation 1, Frank Grosveld
MGI ID: MGI:2449487
Synonyms: Btk-
Gene: Btk  Location: ChrX:134542336-134583570 bp, - strand  Genetic Position: ChrX, 56.18 cM
Germline Transmission:  Earliest citation of germline transmission: J:35769
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
Mutation detailsA vector was constructed that contained an in-frame fusion of 1.5 kb 5' genomic sequences of the targeted gene (up to exon 8) to the nuclear localization signal (nls) of the SV40 T antigen and the E. coli lacZ gene. After homologous recombination in ES cells, only exons 1 - 7 of the targeted gene were intact and were fused to the lacZ reporter. Western blots of total cell lysates from spleen cells and from thymocytes showed no detectable protein for the targeted gene in hemizygous mutant male mice. (J:35769)
View phenotypes and curated references for all genotypes (concatenated display).
Disease models
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Btk Mutation:  15 strains or lines available
Original:  J:35769 Hendriks RW, et al., Inactivation of Btk by insertion of lacZ reveals defects in B cell development only past the pre-B cell stage. EMBO J. 1996 Sep 16;15(18):4862-72
All:  24 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory