Tg(TAG-Tcra,TAG-Tcrb)1Dh
Transgene Detail

Summary

• Symbol: Tg(TAG-Tcra,TAG-Tcrb)1Dh
• Name: transgene insertion 1, Douglas Hanahan
• MGI ID: MGI:2449060
• Synonyms: Tag-TCR1, TCR1
• Transgene: Tg(TAG-Tcra,TAG-Tcrb)1Dh Location: unknown
• Alliance: Tg(TAG-Tcra,TAG-Tcrb)1Dh page

Transgene origin

• Strain of Origin: (C57BL/6 x DBA/2)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(TAG-Tcra,TAG-Tcrb)1Dh expresses 2 genes
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Tcra (MGI:98553)
  mouse	Tcrb (MGI:98578)

• Mutation details: Two transgene constructs were used in the generation of this allele. One construct consists of a Tcra sequence encoding the Valpha2JalphaMT1-27 rearrangement, 6 kb of 5' flanking sequence, 3.8 kb of 3' flanking sequence, and a neomycin resistance gene. The other construct consists of a Tcrb sequence encoding the Vbeta8.3DJbeta2.4 rearrangement, 5.1 kb of 5' flanking sequence, 0.4 kb of 3' flanking sequence, and a neomycin resistance gene. This transgenic line carries two copies of the Tcrb construct and one copy of the Tcra construct cointegrated at one locus. (J:81330)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This allele is refered to as Tag-TCR1 by the authors. Tag-TCR1 animals carry 2 copies of Tcrb and 1 copy of Tcra integrated into the same locus.

References

• Original: J:81330 Forster I, et al., Limited capacity for tolerization of CD4+ T cells specific for a pancreatic beta cell neo-antigen. Immunity. 1995 Jun;2(6):573-85
• All: 3 reference(s)