Brca2^tm1Kamc
Targeted Allele Detail

Nomenclature

• Symbol: Brca2^tm1Kamc
• Name: breast cancer 2, early onset; targeted mutation 1, Kimberly A McAllister
• MGI ID: MGI:2448890
• Synonyms: Brca2^delta27
• Gene: Brca2 Location: Chr5:150522630-150570329 bp, + strand Genetic Position: Chr5, 89.52 cM

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:75026
• Parent Cell Line: BK4 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: LoxP sites flanking exon 27 and a PGK-neo cassette were introduced via homologous recombination. Exon 27 was deleted in correctly targeted ES cells by transient expression of cre-recombinase. This exon encodes the C-terminal domain, which interacts with Rad51, contains a nuclear localization signal, and is required for maintenence of genomic stability in the event of DNA damage. RT-PCR analysis of testes RNA from mutant animals using exon 27-specific primers did not detect transcripts. However, authors were unable to confirm absence of protein expression because antibodies specific to this protein are currently not available. (J:75026)

Disease models

Expression

In Structures Affected by this Mutation:

4 anatomical structures

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Brca2 Mutation: 76 strains or lines available

References

• Original: J:75026 McAllister KA, et al., Cancer susceptibility of mice with a homozygous deletion in the COOH-terminal domain of the Brca2 gene. Cancer Res. 2002 Feb 15;62(4):990-4
• All: 9 reference(s)