Brca2tm1Kamc
Targeted Allele Detail
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Symbol: |
Brca2tm1Kamc |
Name: |
breast cancer 2, early onset; targeted mutation 1, Kimberly A McAllister |
MGI ID: |
MGI:2448890 |
Synonyms: |
Brca2delta27 |
Gene: |
Brca2 Location: Chr5:150522630-150570329 bp, + strand Genetic Position: Chr5, 89.52 cM
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Germline Transmission: |
Earliest citation of germline transmission:
J:75026
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Parent Cell Line: |
BK4 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Null/knockout) |
Mutations: |
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Insertion, Intragenic deletion
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Mutation details: LoxP sites flanking exon 27 and a PGK-neo cassette were introduced via homologous recombination. Exon 27 was deleted in correctly targeted ES cells by transient expression of cre-recombinase. This exon encodes the C-terminal domain, which interacts with Rad51, contains a nuclear localization signal, and is required for maintenence of genomic stability in the event of DNA damage. RT-PCR analysis of testes RNA from mutant animals using exon 27-specific primers did not detect transcripts. However, authors were unable to confirm absence of protein expression because antibodies specific to this protein are currently not available.
(J:75026)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:75026 McAllister KA, et al., Cancer susceptibility of mice with a homozygous deletion in the COOH-terminal domain of the Brca2 gene. Cancer Res. 2002 Feb 15;62(4):990-4 |
All: |
10 reference(s) |
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