Tg(Cd4-CUL1-N252)1Ingh
Transgene Detail

Summary

• Symbol: Tg(Cd4-CUL1-N252)1Ingh
• Name: transgene insertion 1, Giorgio Inghirami
• MGI ID: MGI:2447878
• Synonyms: Tg-Cul1-N252
• Transgene: Tg(Cd4-CUL1-N252)1Ingh Location: unknown
• Alliance: Tg(Cd4-CUL1-N252)1Ingh page

Transgene origin

• Strain of Origin: Not Specified

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Cd4-CUL1-N252)1Ingh expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  CUL1 (8454)

  Cul1	(MGI:1349658)

• Mutation details: The transgene consists of a mouse Cd4 promoter and a flag-tagged mutant human CUL1 sequence encoding 252 N-terminal residues. Mouse Cd4 regulatory sequences, including the minimal enhancer, minimal promoter, 70 bp of exon 1, and part of intron 1, were used to drive transgene expression in T lymphocytes. The Cd4 promoter lacks the silencer region and is transcriptionally active in both single- and double-positive T cells. Transgene expression was confirmed by Western blot analysis of thymocyte extracts from transgenic animals. The transgenic human protein was shown to associate with mouse Skp1 protein via immunoprecipitation immunoblot. (J:80320)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Three transgenic lines (4, 10, and 20) were created. Highest expressing lines 10 and 20 were used for analysis.

References

• Original: J:80320 Piva R, et al., In vivo interference with Skp1 function leads to genetic instability and neoplastic transformation. Mol Cell Biol. 2002 Dec;22(23):8375-87
• All: 2 reference(s)