Mmp20tm1Jdb
Targeted Allele Detail
|
Symbol: |
Mmp20tm1Jdb |
Name: |
matrix metallopeptidase 20 (enamelysin); targeted mutation 1, John D Bartlett |
MGI ID: |
MGI:2447874 |
Synonyms: |
enamelysin- |
Gene: |
Mmp20 Location: Chr9:7628232-7674969 bp, + strand Genetic Position: Chr9, 2.46 cM
|
Alliance: |
Mmp20tm1Jdb page
|
|
Enamel delaminates from the dentin surface in Mmp20tm1Jdb/Mmp20tm1Jdb mouse molars
Show the 3 phenotype image(s) involving this allele.
|
|
|
Germline Transmission: |
Earliest citation of germline transmission:
J:80925
|
Parent Cell Line: |
HM-1 (ES Cell)
|
Strain of Origin: |
129P2/OlaHsd-Hprt1b-m3
|
|
Allele Type: |
|
Targeted (Null/knockout) |
Mutations: |
|
Insertion, Intragenic deletion
|
|
|
Mutation details: Sequence encompassing a portion of intron 4 and all of exon 5 was replaced with a Hprt minigene inserted by homologous recombination. The deleted region encoded the conserved zinc-binding site of the catalytic domain. Northern blot analysis of total RNA obtained from homozygous mutant incisor homogenates indicated a lack of transcript containing exon 5. Enamelysin activity was undetected by zymography of proteins isolated from molars of postnatal homozygous mutant mice.
(J:80925)
|
|
|
View phenotypes and curated references for all genotypes (concatenated display).
|
|
|
|
Original: |
J:80925 Caterina JJ, et al., Enamelysin (matrix metalloproteinase 20)-deficient mice display an amelogenesis imperfecta phenotype. J Biol Chem. 2002 Dec 20;277(51):49598-604 |
All: |
15 reference(s) |
|