Tg(Col2a1-Runx2*)3Tok
Transgene Detail

Summary

• Symbol: Tg(Col2a1-Runx2*)3Tok
• Name: transgene insertion 3, Toshihisa Komori
• MGI ID: MGI:2447359
• Synonyms: Col2a1-DN-Cbfa1, Tg(Col2a1-Runx2DN)3Tok
• Transgene: Tg(Col2a1-Runx2*)3Tok Location: unknown
• Alliance: Tg(Col2a1-Runx2*)3Tok page

Transgene origin

• Strain of Origin: (C57BL/6 x C3H)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Col2a1-Runx2*)3Tok expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Runx2 (MGI:99829)

• Mutation details: A cDNA fragment encoding the runt domain of mouse Runx2 was cloned between the promoter and enhancer elements of Col2a1 (active in chondrocytes) to produce a dominant negative transgene. Transgene expression was confirmed by Northern blot analysis of RNA extracted from the head or trunk of day E12.5 transgenic embryos. (J:68793)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

References

• Original: J:68793 Ueta C, et al., Skeletal malformations caused by overexpression of Cbfa1 or its dominant negative form in chondrocytes. J Cell Biol. 2001 Apr 2;153(1):87-100
• All: 1 reference(s)