Tg(INSR)2Ebna
Transgene Detail

Summary

• Symbol: Tg(INSR)2Ebna
• Name: transgene insertion 2, Yousuke Ebna
• MGI ID: MGI:2446614
• Synonyms: hIR^K1030M(+), Insr^K1030M
• Transgene: Tg(INSR)2Ebna Location: unknown
• Alliance: Tg(INSR)2Ebna page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(INSR)2Ebna expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  INSR (3643)

  Insr	(MGI:96575)

• Mutation details: A transgenic construct was engineered to contain human insulin receptor sequence modified to contain a lysine to methionine missense mutation at codon 1030. The substitution of lysine 1030 with methionine is expected to disrupt the putative ATP binding site, thereby eliminating the tyrosine kinase activity of the protein. A 1.5 kb region of the human insulin receptor (INSR) promoter was included to direct expression in a variety of tissues. The expression of the human transcript in transgenic mice was verified by Northern blot analysis. (J:80642)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Founder mice contained 2 to 3 copies of the transgenic construct.

References

• Original: J:80642 Nishiyama T, et al., Expression of the gene encoding the tyrosine kinase-deficient human insulin receptor in transgenic mice. Gene. 1994 Apr 20;141(2):187-92
• All: 3 reference(s)