Tg(Mylk2-Ucpd1)1Sem
Transgene Detail

Summary

• Symbol: Tg(Mylk2-Ucpd1)1Sem
• Name: transgene insertion 1, Clay F Semenkovich
• MGI ID: MGI:2446457
• Synonyms: Ucp-L
• Transgene: Tg(Mylk2-Ucpd1)1Sem Location: unknown
• Alliance: Tg(Mylk2-Ucpd1)1Sem page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F1

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Mylk2-Ucpd1)1Sem expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ucp1 (MGI:98894)

• Mutation details: A 1 kb mouse Ucp1 cDNA under the control of a rat Mylk2 promoter was used for the transgene. A human ALB intron and human GAPD termination and polyadenylation signal were added to the mouse sequence. RT-PCR analysis confirmed transgene expression in skeletal muscle and no other tissues examined. (J:65242)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

Two transgenic founder lines were established, a low expressing line named Ucp-L and a high expressing line named Ucp-H.

References

• Original: J:65242 Li B, et al., Skeletal muscle respiratory uncoupling prevents diet-induced obesity and insulin resistance in mice. Nat Med. 2000 Oct;6(10):1115-20
• All: 3 reference(s)