Gt(ROSA)26Sortm1(cre/ERT)Brn
Targeted Allele Detail
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Symbol: |
Gt(ROSA)26Sortm1(cre/ERT)Brn |
Name: |
gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Anton Berns |
MGI ID: |
MGI:2445311 |
Synonyms: |
ACM-cre, Cre-ERTm, Gt(ROSA)26Sortm1(cre/Esr1)Brn, R26cre-ERT, R26-CreERT, R26Cre-ERT, Tx-Cre |
Gene: |
Gt(ROSA)26Sor Location: Chr6:113044389-113054205 bp, - strand Genetic Position: Chr6, 52.73 cM
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Alliance: |
Gt(ROSA)26Sortm1(cre/ERT)Brn page
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Germline Transmission: |
Earliest citation of germline transmission:
J:80160
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Parent Cell Line: |
E14 (ES Cell)
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Strain of Origin: |
129P2/OlaHsd
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Allele Type: |
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Targeted (Inducible, Recombinase) |
Inducer: |
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tamoxifen |
Mutation: |
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Insertion
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Gt(ROSA)26Sortm1(cre/ERT)Brn expression driven by
1 gene
Knock-in expression driven by:
Organism |
Driver Gene |
Note |
mouse |
Gt(ROSA)26Sor (MGI:104735) |
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Mutation details: The endogenous locus was modified by insertion of a construct containing the rabbit beta-globin 3' splice site followed by the cre/ERT fusion gene, which encodes Cre recombinase joined to the G321R mutant hormone-binding domain of the human estrogen receptor that is activated by 4-hydroxytamoxifen (4-OHT), but not by the natural ER ligand 17-beta-estradiol. The fusion gene is followed by the rabbit beta-globin polyadenylation signal and a PGK-puro (puromycin resistance) cassette. Efficient Cre-mediated recombination in transgenic mice carrying floxed reporter genes was reported to reflect constitutive expression of the Gt(ROSA)26Sor from preimplantation stages onward.
(J:80160)
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Activity: |
Tissue activity of this recombinase allele
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Driver:
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Gt(ROSA)26Sor
(mouse)
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View phenotypes and curated references for all genotypes (concatenated display).
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Original: |
J:80160 Vooijs M, et al., A highly efficient ligand-regulated Cre recombinase mouse line shows that LoxP recombination is position dependent. EMBO Rep. 2001 Apr;2(4):292-7 |
All: |
70 reference(s) |
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