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Tnftm2Gkl
Targeted Allele Detail
Nomenclature
Symbol: Tnftm2Gkl
Name: tumor necrosis factor; targeted mutation 2, George Kollias
MGI ID: MGI:2388715
Synonyms: TNFdeltaARE
Gene: Tnf  Location: Chr17:35199381-35202007 bp, - strand  Genetic Position: Chr17, 18.59 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:54056
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details69 bp encompassing the AU-rich elements (ARE) were deleted by homologous recombination of a floxed neo cassette and subsequent in vitro cre mediated excision. The resultant allele contained a single loxP site in place of the ARE sequence. ELISA of homozygous mutant mice showed protein levels higher than in wild-type mice, indicating a suppressive role of the AREs. (J:54056)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Disease models
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Expression
In Structures Affected by this Mutation: 12 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Tnf Mutation:  35 strains or lines available
References
Original:  J:54056 Kontoyiannis D, et al., Impaired on/off regulation of TNF biosynthesis in mice lacking TNF AU- rich elements: implications for joint and gut-associated immunopathologies. Immunity. 1999 Mar;10(3):387-98
All:  33 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
04/16/2019
MGI 6.13
The Jackson Laboratory