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Trp53tm2Mok
Targeted Allele Detail
Nomenclature
Symbol: Trp53tm2Mok
Name: transformation related protein 53; targeted mutation 2, Motoya Katsuki
MGI ID: MGI:2388703
Synonyms: p53-, Trp53tm1.1Mok
Gene: Trp53  Location: Chr11:69580359-69591873 bp, + strand  Genetic Position: Chr11, 42.83 cM, cytoband B2-C
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:79629
Parent Cell Line:  CCE/EK.CCE (ES Cell)
Strain of Origin:  129S/SvEv-Gpi1c
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsThe gene was disrupted using a two-step targeting strategy. First, exons 2-10 were replaced with a neo-TK cassette via homologous recombination. Next, correctly targeted ES cells were subjected to another round of gene targeting to replace the neo-TK cassette with a lacZ cassette. Successful gene replacement was selected for via gancyclovir resistance. Homozygous mutant animals were successfully established. (J:79629)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 1 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Trp53 Mutation:  155 strains or lines available
References
Original:  J:79629 Gondo Y, et al., Gene replacement of the p53 gene with the lacZ gene in mouse embryonic stem cells and mice by using two steps of homologous recombination. Biochem Biophys Res Commun. 1994 Jul 29;202(2):830-7
All:  7 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/19/2020
MGI 6.15
The Jackson Laboratory