Hoxa3^tm1Kth
Targeted Allele Detail

Summary

• Symbol: Hoxa3^tm1Kth
• Name: homeobox A3; targeted mutation 1, Kirk R Thomas
• MGI ID: MGI:2388564
• Synonyms: a3^lox, Hox3a^null
• Gene: Hoxa3 Location: Chr6:52146042-52190316 bp, - strand Genetic Position: Chr6, 25.4 cM
• Alliance: Hoxa3^tm1Kth page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79620
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutation: Insertion
• Mutation details: A self-excising neomycin resistance cassette was inserted into the homeodomain of Hoxa3. The targeting construct is composed of a mouse testes-specific Ace promoter fused to the cre recombinase and neomycin resistance genes. The entire construct is flanked by loxP sites and undergoes self-excision during spermiogenesis, leaving behind a single loxP insertion sequence. (J:79620)

Expression

In Structures Affected by this Mutation:

2 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Hoxa3 Mutation: 24 strains or lines available

References

• Original: J:79620 Bunting M, et al., Targeting genes for self-excision in the germ line. Genes Dev. 1999 Jun 15;13(12):1524-8
• All: 2 reference(s)