Tg(Lck-ZAP70-Y319F)2Achn
Transgene Detail

Summary

• Symbol: Tg(Lck-ZAP70-Y319F)2Achn
• Name: transgene insertion 2, Andrew C Chan
• MGI ID: MGI:2388336
• Synonyms: line 974, ZAP-70(Y319F)
• Transgene: Tg(Lck-ZAP70-Y319F)2Achn Location: unknown
• Alliance: Tg(Lck-ZAP70-Y319F)2Achn page

Transgene origin

• Strain of Origin: C57BL/6

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Lck-ZAP70-Y319F)2Achn expresses 1 gene
  Transgene expresses:
  

  Organism

  Expressed Gene

  Homolog in Mouse

  Note

  human

  ZAP70 (7535)

  Zap70

  (MGI:99613)

  Transgene codes for a protein with a tyrosine to phenylalanine mutation.

• Mutation details: The human ZAP70 gene carrying a tyrosine to phenylalanine substitution at position 319 (Y319F) was used as the transgene. The transgene is expressed in thymocytes under the control of mouse Lck promoter and contains a hemagglutinin epitope tag at the N-terminal end and a human growth hormone polyA tail. Transgene expression was verified by Western blot analysis. (J:71098)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available

Notes

This transgenic founder (line 974) expresses 4-fold higher levels of transgene product compared to other transgenic lines.

References

• Original: J:71098 Gong Q, et al., Requirement for tyrosine residues 315 and 319 within zeta chain-associated protein 70 for T cell development. J Exp Med. 2001 Aug 20;194(4):507-18
• All: 1 reference(s)