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Targeted Allele Detail
Symbol: Raf1tm1Bacc
Name: v-raf-leukemia viral oncogene 1; targeted mutation 1, Manuela Baccarini
MGI ID: MGI:2388026
Synonyms: c-raf-1-, Raf-1-
Gene: Raf1  Location: Chr6:115618569-115676635 bp, - strand  Genetic Position: Chr6, 53.62 cM, cytoband C3
Germline Transmission:  Earliest citation of germline transmission: J:69056
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsExon 3 was flanked with loxP sites by insertion of a floxed TK-neo cassette and a single loxP site into the upstream and downstream introns, respectively. Exon 3 was removed by transient expression of cre recombinase in correctly targeted ES cells. Absence of gene expression was confirmed by Western blot analysis of mouse embryonic fibroblasts (MEFs) derived from homozygous mutant embryos. Immune complex kinase assays showed a lack of enzyme activity in MEFs from homozygous mutants. (J:69056)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 6 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Raf1 Mutation:  82 strains or lines available
Original:  J:69056 Mikula M, et al., Embryonic lethality and fetal liver apoptosis in mice lacking the c-raf-1 gene. EMBO J. 2001 Apr 17;20(8):1952-62
All:  6 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
MGI 6.16
The Jackson Laboratory