Rbpjl^tm1Hon
Targeted Allele Detail

Summary

• Symbol: Rbpjl^tm1Hon
• Name: recombination signal binding protein for immunoglobulin kappa J region-like; targeted mutation 1, Tasuku Honjo
• MGI ID: MGI:2387866
• Gene: Rbpjl Location: Chr2:164245061-164257368 bp, + strand Genetic Position: Chr2, 85.16 cM
• Alliance: Rbpjl^tm1Hon page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:59458
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout, Reporter)
• Mutation: Insertion
• Mutation details: The first 19 bp of exon 1 were fused in-frame to a lacZ-PGK-neo construct and introduced to the gene via homologous recombination. Northern blot analysis of lung RNA from homozygous mutants using primers to exons 8 and 9 revealed a near complete loss of gene transcription. RT-PCR analysis using primers to exon 2 detected weak expression in lungs from homozygous mutants leading the investigators to believe there may be an additional promoter downstream of exon 1. The lacZ fusion product is expressed properly in the brain as demonstrated by in situ hybridization. (J:59458)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Rbpjl Mutation: 25 strains or lines available

References

• Original: J:59458 Minoguchi S, et al., Studies on the cell-type specific expression of RBP-L, a RBP-J family member, by replacement insertion of beta-galactosidase. J Biochem. 1999 Oct;126(4):738-47
• All: 1 reference(s)