St6gal1^tm1Jxm
Targeted Allele Detail

Summary

• Symbol: St6gal1^tm1Jxm
• Name: beta galactoside alpha 2,6 sialyltransferase 1; targeted mutation 1, Jamey Marth
• MGI ID: MGI:2387745
• Synonyms: ST6Gal^delta
• Gene: St6gal1 Location: Chr16:23043490-23179100 bp, + strand Genetic Position: Chr16, 14.03 cM
• Alliance: St6gal1^tm1Jxm page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:79166
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exon 2 was flanked by loxP sites and a loxP-tk-neo cassette was inserted into the upstream intron via homologous recombination. Transient expression of cre recominase in correctly targeted cells facilitated the removal of exon 2 and the loxP-tk-neo cassette. The mutation deletes the cytoplasmic, tranmembrane, and the majority of the catalytic domains. Enzymatic analysis of liver and spleen extracts from homozygous mutant animals showed no detectable enzyme activity. (J:79166)

Tumor Data

List all tumor models in MMHCdb carrying St6gal1^tm1Jxm

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any St6gal1 Mutation: 55 strains or lines available

References

• Original: J:79166 Hennet T, et al., Immune regulation by the ST6Gal sialyltransferase. Proc Natl Acad Sci U S A. 1998 Apr 14;95(8):4504-9
• All: 23 reference(s)