Tg(Hoxb7-Ret)1Cos
Transgene Detail

Summary

• Symbol: Tg(Hoxb7-Ret)1Cos
• Name: transgene insertion 1, Frank Costantini
• MGI ID: MGI:2387447
• Synonyms: Hoxb7/RET
• Transgene: Tg(Hoxb7-Ret)1Cos Location: unknown
• Alliance: Tg(Hoxb7-Ret)1Cos page

Transgene origin

• Strain of Origin: (C57BL/6 x CBA)F2

Transgene description

• Transgene Type: Transgenic (Inserted expressed sequence)
• Mutation: Insertion
• Tg(Hoxb7-Ret)1Cos expresses 1 gene
  Transgene expresses:
  

  Organism	Expressed Gene	Note
  mouse	Ret (MGI:97902)

• Mutation details: The transgene consists of a mouse Ret cDNA encoding the RET9 isoform fused to mouse Hoxb7 promoter sequences (-1316 to +81). Mouse Hoxb7 promoter was used to drive expression of the transgene throughout the uretic bud branches. The construct used contained a deletion of all but 20 bp of the 5' non-coding region of Ret9 eliminating an out-of-frame ATG located at position 169 upstream from the initiation codon at position 201. This truncation may have increased the translational efficiency of Ret9 mRNA leading to variable dominant renal defects observed in some transgenic mice. Expression of the transgene was confirmed by Northern blot analysis of brain and kidney samples from transgenic animals. (J:52988)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available

Notes

Three transgenic lines (Tg5, Tg6, Tg8) expressing the highest levels of the transgene were used for analysis.

References

• Original: J:52988 Srinivas S, et al., Dominant effects of RET receptor misexpression and ligand-independent RET signaling on ureteric bud development. Development. 1999 Apr;126(7):1375-86
• All: 3 reference(s)