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Blktm1Tara
Targeted Allele Detail
Nomenclature
Symbol: Blktm1Tara
Name: B lymphoid kinase; targeted mutation 1, Alexander Tarakhovsky
MGI ID: MGI:2386868
Synonyms: blk-
Gene: Blk  Location: Chr14:63372837-63417187 bp, - strand  Genetic Position: Chr14, 33.25 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:60221
Parent Cell Line:  E14.1 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsSequences encoding the essential portion of the kinase domain in exon 8 were replaced with a neomycin resistance gene via homologous recombination. Gene expression was not detected in splenic B-cells from homozygous mutant animals via Northern blot, but RT-PCR analysis detected transcripts containing exons 1-4. However, Western blot using antibodies directed against the N-terminal region of the protein did not detect any translation product from the truncated transcript. (J:60221)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Blk Mutation:  2 strains or lines available
Notes
ES cell line = E14-1.1

References
Original:  J:60221 Texido G, et al., The B-cell-specific Src-family kinase Blk is dispensable for B-cell development and activation. Mol Cell Biol. 2000 Feb;20(4):1227-33
All:  5 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
07/21/2015
MGI 5.22
The Jackson Laboratory