Hif1a^tm3Rsjo
Targeted Allele Detail

Summary

• Symbol: Hif1a^tm3Rsjo
• Name: hypoxia inducible factor 1, alpha subunit; targeted mutation 3, Randall S Johnson
• MGI ID: MGI:2386679
• Synonyms: +^f, Hif1a^2lox, HIF1a^f+, Hif1a^fl, Hif1a^loxP, Hif-1alpha 2-lox, HIF-1alpha^+f, HIF-1alpha^F, HIF1alpha^flox, Hif^+f, Hif^f, I.1-^lox
• Gene: Hif1a Location: Chr12:73948149-73994304 bp, + strand Genetic Position: Chr12, 31.99 cM
• Alliance: Hif1a^tm3Rsjo page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78980
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Conditional ready, No functional change)
• Mutation: Insertion
• Mutation details: A loxP site was inserted in intron 1 and a floxed neomycin resistance cassette was placed in intron 2. Exon 2 was left flanked by loxP sites after the neo cassette was excised by in vitro expression of cre recombinase. (J:78980)

Disease models

Expression

In Mice Carrying this Mutation:	13 assay results 6 RNA-Seq or microarray experiment(s)
In Structures Affected by this Mutation:	35 anatomical structure(s)

Tumor Data

List all tumor models in MMHCdb carrying Hif1a^tm3Rsjo

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 3 strains available Cell Lines: 0 lines available
• Carrying any Hif1a Mutation: 48 strains or lines available

References

• Original: J:78980 Ryan HE, et al., Hypoxia-inducible factor-1alpha is a positive factor in solid tumor growth. Cancer Res. 2000 Aug 1;60(15):4010-5
• All: 422 reference(s)