Nr1i2^tm1Rev
Targeted Allele Detail

Summary

• Symbol: Nr1i2^tm1Rev
• Name: nuclear receptor subfamily 1, group I, member 2; targeted mutation 1, Ronald M Evans
• MGI ID: MGI:2386087
• Synonyms: PXR^-, PXR-null
• Gene: Nr1i2 Location: Chr16:38068711-38115211 bp, - strand Genetic Position: Chr16, 26.64 cM
• Alliance: Nr1i2^tm1Rev page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:63639
• Parent Cell Line: J1 (ES Cell)
• Strain of Origin: 129S4/SvJae

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: Exons 2 and 3 encoding amino acids 63-170 of the DNA-binding domain were replaced with a PGK-neo cassette via homologous recombination. Northern blot analysis confirmed the absence of gene expression in the liver and small intestine of homozygous mutant animals. (J:63639)

Expression

In Mice Carrying this Mutation:

7 assay results

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Nr1i2 Mutation: 33 strains or lines available

References

• Original: J:63639 Xie W, et al., Humanized xenobiotic response in mice expressing nuclear receptor SXR. Nature. 2000 Jul 27;406(6794):435-9
• All: 31 reference(s)