Serpinh1^tm1Kzn
Targeted Allele Detail

Summary

• Symbol: Serpinh1^tm1Kzn
• Name: serine (or cysteine) peptidase inhibitor, clade H, member 1; targeted mutation 1, Kazuhiro Nagata
• MGI ID: MGI:2385994
• Synonyms: Hsp47^-
• Gene: Serpinh1 Location: Chr7:98994583-99002321 bp, - strand Genetic Position: Chr7, 54.07 cM
• Alliance: Serpinh1^tm1Kzn page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:64899
• Parent Cell Line: E14 (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: The gene was disrupted by replacement of exon 4 and part of exon 5 with a neomycin resistance cassette via homologous recombination. Homozygous mutant embryos were detected by Southern blot up to day E10.5. Homozygous mutant embryos lacked gene expression as demonstrated by RT-PCR and Western blot analysis. (J:64899)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 1 strain available Cell Lines: 0 lines available
• Carrying any Serpinh1 Mutation: 59 strains or lines available

References

• Original: J:64899 Nagai N, et al., Embryonic lethality of molecular chaperone hsp47 knockout mice is associated with defects in collagen biosynthesis. J Cell Biol. 2000 Sep 18;150(6):1499-506
• All: 3 reference(s)