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Targeted Allele Detail
Symbol: Rb1tm2Tyj
Name: RB transcriptional corepressor 1; targeted mutation 2, Tyler Jacks
MGI ID: MGI:2385637
Gene: Rb1  Location: Chr14:73192858-73325951 bp, - strand  Genetic Position: Chr14, 38.73 cM, cytoband D3
Mouse Generated:  Earliest citation of chimera generation: J:59268
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsInsertion of a hygromycin resistance cassette into one allele of the Rb1 gene replaced part of intron 3 and introduced three nucleotide changes into exon 3, creating two termination codons and a new PstI site. This was accomplished in ES cells in which Rb1 had already been targeted. The resulting ES cells are Rb1tm1Tyj/Rb1tm2Tyj. (J:59268)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 3 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Rb1 Mutation:  78 strains or lines available
Homozygous mutants at this locus are embryonic lethal. ES cells homozygous for functional knockout of this locus were created so that phenotypes resulting from homozygous cells could be studied in adult chimeras. The contribution of double knockout cells to the composition of chimeric mice varied from 30 to 60%.
Original:  J:59268 Williams BO, et al., Extensive contribution of Rb-deficient cells to adult chimeric mice with limited histopathological consequences. EMBO J. 1994 Sep 15;13(18):4251-9
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.16
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