Pik3cd^tm1.1Bvan
Targeted Allele Detail

Summary

• Symbol: Pik3cd^tm1.1Bvan
• Name: phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic subunit delta; targeted mutation 1.1, Bart Vanhaesebroeck
• MGI ID: MGI:2385596
• Synonyms: P110delta^D910A, P110delta KI, PI3Kdeltal^D910A
• Gene: Pik3cd Location: Chr4:149733625-149787023 bp, - strand Genetic Position: Chr4, 80.15 cM
• Alliance: Pik3cd^tm1.1Bvan page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:78412
• Parent Cell Line: E14TG2a (ES Cell)
• Strain of Origin: 129P2/OlaHsd

Mutation description

• Allele Type: Targeted
• Mutation: Nucleotide substitutions
• Mutation details: This mutation is derived from Pik3cd^tm1Bvan. Chimeric animals carrying Pik3cd^tm1Bvan were crossed to a cre-expressing line to remove the floxed lacZ-neo cassette. The D910A mutation is left intact. Western blot analysis demonstrated the expression of mutant protein in thymocytes is similar to that of wild-type protein. Immunoprecipitates from homozygous mutant animals showed that mutant proteins were completely devoid of kinase activity. (J:78412)

Expression

In Structures Affected by this Mutation:

8 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Pik3cd Mutation: 43 strains or lines available

References

• Original: J:78412 Okkenhaug K, et al., Impaired B and T cell antigen receptor signaling in p110delta PI 3-kinase mutant mice. Science. 2002 Aug 9;297(5583):1031-4
• All: 72 reference(s)