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Targeted Allele Detail
Symbol: Gfaptm4Ito
Name: glial fibrillary acidic protein; targeted mutation 4, Shigeyoshi Itohara
MGI ID: MGI:2384721
Synonyms: Gfaphm3
Gene: Gfap  Location: Chr11:102887336-102897200 bp, - strand  Genetic Position: Chr11, 66.48 cM, cytoband D
Germline Transmission:  Earliest citation of germline transmission: J:78341
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted
Mutations:    Insertion, Intragenic deletion, Nucleotide substitutions
Mutation detailsThe 5' portion of exon 1 was replaced with corresponding human sequence mutagenized to contain substitution mutations at all 5 phosphorylation sites, Thr7, Ser8, Ser13, Ser17, and Ser38 (human positions). The incorporation of the specific mutations was verified by sequence analysis as well as by Western blot analysis using antibodies directed toward the phosphorylation sites. Northern blot analysis of total brain RNA showed that transcript levels were unaffected in mutant mice. Immunohistochemical analysis of brain tissue showed that while the distribution of protein was conserved in mutant mice, protein levels were reduced in various regions. (J:78341)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 2 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Gfap Mutation:  22 strains or lines available
Original:  J:78341 Takemura M, et al., Protective role of phosphorylation in turnover of glial fibrillary acidic protein in mice. J Neurosci. 2002 Aug 15;22(16):6972-9
All:  1 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
The Jackson Laboratory