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Nfatc1tm2Glm
Targeted Allele Detail
Nomenclature
Symbol: Nfatc1tm2Glm
Name: nuclear factor of activated T cells, cytoplasmic, calcineurin dependent 1; targeted mutation 2, Laurie H Glimcher
MGI ID: MGI:2384395
Gene: Nfatc1  Location: Chr18:80606205-80713071 bp, - strand  Genetic Position: Chr18, 53.66 cM, cytoband E4
Mutation
origin
Mouse Generated:  Earliest citation of chimera generation: J:78376
Parent Cell Line:  D3 (ES Cell)
Strain of Origin:  129S2/SvPas
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsSequence encoding 19 amino acids (635-653) of the Rel-homology domain were replaced with a hygromycin selection cassette. A frameshift mutation was introduced into the downstream sequence encoding amino acids 654 through 710. Western blot analysis showed the presence of truncated peptide in homozygous mutant mice. Electromobility shift assays showed a lack of DNA-binding activity. Generated in ES cells already carrying the Nfatc1tm2Glm allele on the other chromosome. These mice were studied as chimeras made in blastocysts homozygous for an unspecified Rag2 knockout (J:78376)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Nfatc1 Mutation:  18 strains or lines available
Notes
Generated in ES cells already carrying the Nfatc1tm2Glm allele on the other chromosome. These mice were studied as chimeras made in blastocysts homozygous for an unspecified Rag2 knockout.
References
Original:  J:78376 Ranger AM, et al., Delayed lymphoid repopulation with defects in IL-4-driven responses produced by inactivation of NF-ATc. Immunity. 1998 Jan;8(1):125-34
All:  1 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
08/17/2016
MGI 6.05
The Jackson Laboratory