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Targeted Allele Detail
Symbol: Irak3tm1Flv
Name: interleukin-1 receptor-associated kinase 3; targeted mutation 1, Richard A Flavell
MGI ID: MGI:2384197
Synonyms: IRAK-M-
Gene: Irak3  Location: Chr10:120141648-120202130 bp, - strand  Genetic Position: Chr10, 67.94 cM, cytoband D2
Small size and reduced total bone mineral density in Irak3tm1Flv/Irak3tm1Flv mice

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:78189
Parent Cell Line:  W9.5/W95 (ES Cell)
Strain of Origin:  129S1/Sv-Oca2+ Tyr+ Kitl+
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation details1.2 kb of sequence were replaced with a floxed neo cassette. The deleted region contained 3 exons encoding two thirds of the kinase domain. While Western blot analysis of LPS-stimulated bone marrow macrophage extracts using a carboxy terminal antibody confirmed the absence of normal protein in homozygous mutant mice, truncated transcript was detected by Northern blot analysis using an amino terminal probe. Sequence analysis of RT-PCR products revealed an in frame stop codon generated by aberrant splicing of the endogenous gene and the neo transgene. (J:78189)
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 2 strains available      Cell Lines: 0 lines available
Carrying any Irak3 Mutation:  76 strains or lines available
Original:  J:78189 Kobayashi K, et al., IRAK-M is a negative regulator of Toll-like receptor signaling. Cell. 2002 Jul 26;110(2):191-202
All:  15 reference(s)

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Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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MGI 6.01
The Jackson Laboratory