Shc1^tm1Paw
Targeted Allele Detail

Summary

• Symbol: Shc1^tm1Paw
• Name: src homology 2 domain-containing transforming protein C1; targeted mutation 1, Anthony Pawson
• MGI ID: MGI:2384189
• Synonyms: ShcA^deltaex2/3
• Gene: Shc1 Location: Chr3:89325858-89337336 bp, + strand Genetic Position: Chr3, 39.11 cM
• Alliance: Shc1^tm1Paw page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:62182
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: 2.0 kb of sequence, including exons 2 and 3, was replaced with a neomycin selection cassette. The deleted region contained the start codons for the three known isoforms and encoded portions of the PTB domain in each. Though transcript was undetected in homozygous mutant mice, a truncated protein (40 kD) was identified by Western blot analysis of total embryonic RNA. RT-PCR and in vitro transcription/translation analysis showed aberrant splicing involving the neo transgene and initiation of translation to be at codon 134. (J:62182)

Expression

In Structures Affected by this Mutation:

11 anatomical structure(s)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Shc1 Mutation: 66 strains or lines available

References

• Original: J:62182 Lai KM, et al., The ShcA phosphotyrosine docking protein sensitizes cardiovascular signaling in the mouse embryo. Genes Dev. 2000 May 1;14(9):1132-45
• All: 2 reference(s)