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Shc1tm1Paw
Targeted Allele Detail
Nomenclature
Symbol: Shc1tm1Paw
Name: src homology 2 domain-containing transforming protein C1; targeted mutation 1, Anthony Pawson
MGI ID: MGI:2384189
Synonyms: ShcAdeltaex2/3
Gene: Shc1  Location: Chr3:89418551-89430029 bp, + strand  Genetic Position: Chr3, 39.11 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:62182
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation details2.0 kb of sequence, including exons 2 and 3, was replaced with a neomycin selection cassette. The deleted region contained the start codons for the three known isoforms and encoded portions of the PTB domain in each. Though transcript was undetected in homozygous mutant mice, a truncated protein (40 kD) was identified by Western blot analysis of total embryonic RNA. RT-PCR and in vitro transcription/translation analysis showed aberrant splicing involving the neo transgene and initiation of translation to be at codon 134. (J:62182)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Expression
In Structures Affected by this Mutation: 12 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Shc1 Mutation:  58 strains or lines available
References
Original:  J:62182 Lai KM, et al., The ShcA phosphotyrosine docking protein sensitizes cardiovascular signaling in the mouse embryo. Genes Dev. 2000 May 1;14(9):1132-45
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/26/2020
MGI 6.15
The Jackson Laboratory