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Plp1tm2Wst
Targeted Allele Detail
Nomenclature
Symbol: Plp1tm2Wst
Name: proteolipid protein (myelin) 1; targeted mutation 2, Wilhelm Stoffel
MGI ID: MGI:2384127
Synonyms: dm20/neo
Gene: Plp1  Location: ChrX:136822671-136839733 bp, + strand  Genetic Position: ChrX, 59.1 cM, cytoband F1-F2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:21976
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
 
Mutation details105 bp of exon 3 were deleted to mimic the DM20 isoform and a neomycin resistance cassette was inserted into intron 3 to disrupt splicing of the gene product. Northern blot analysis confirmed the absence of normal transcripts in the brain of homozygous mutant animals. Western blot analysis and silver stained SDS-PAGE gels of myelin samples from homozygous mutants did not detect protein product of either isoform, PLP or DM20. (J:21976)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Plp1 Mutation:  16 strains or lines available
References
Original:  J:21976 Boison D, et al., Disruption of the compacted myelin sheath of axons of the central nervous system in proteolipid protein-deficient mice. Proc Natl Acad Sci U S A. 1994 Nov 22;91(24):11709-13
All:  2 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
05/26/2020
MGI 6.15
The Jackson Laboratory