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Cd3etm1Lov
Targeted Allele Detail
Nomenclature
Symbol: Cd3etm1Lov
Name: CD3 antigen, epsilon polypeptide; targeted mutation 1, Paul E Love
MGI ID: MGI:2384120
Synonyms: CD3eN
Gene: Cd3e  Location: Chr9:44998740-45009627 bp, - strand  Genetic Position: Chr9, 24.84 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:72144
Parent Cell Line:  R1 (ES Cell)
Strain of Origin:  (129X1/SvJ x 129S1/Sv)F1-Kitl+
Mutation
description
Allele Type:    Targeted (Modified regulatory region, Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA 3' portion of exon 5 and all of exon 6, encoding the extracellular and transmembrane domains, were replaced by a floxed neo cassette. Northern blot analysis of total RNA obtained from thymocytes showed an absence of Cd3e transcript and reduced levels of Cd3d and Cd3g transcript in homozygous mutant mice. The downregulation of the delta and gamma polypeptides was believed to be due to the reverse transcriptional orientation of the neo transgene having an effect on the enhancer for Cd3d and Cd3g. To eliminate this effect, Cd3etm1.1Lov was generated by breeding mice possesing this allele with cre expressing mice. (J:72144)
Phenotypes
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View phenotypes and curated references for all genotypes (concatenated display).
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Cd3e Mutation:  12 strains or lines available
References
Original:  J:72144 DeJarnette JB, et al., Specific requirement for CD3epsilon in T cell development. Proc Natl Acad Sci U S A. 1998 Dec 8;95(25):14909-14
All:  14 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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last database update
02/18/2020
MGI 6.14
The Jackson Laboratory