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Nrp2tm1.2Mom
Targeted Allele Detail
Nomenclature
Symbol: Nrp2tm1.2Mom
Name: neuropilin 2; targeted mutation 1.2, Peter Mombaerts
MGI ID: MGI:2183904
Synonyms: Neuropilin-2-Delta, NP2-delta, Nrp2del, Nrp2tm1Mom
Gene: Nrp2  Location: Chr1:62703285-62818695 bp, + strand  Genetic Position: Chr1, 31.35 cM, cytoband C2
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:76686
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Mutation
description
Allele Type:    Targeted (Null/knockout, Reporter)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA loxP site 272 bp upstream of the translation initiation codon was introduced into exon 1 and a loxP-GFP FRT-flanked neo cassette was inserted 1 kb into intron 1 via homologous recombination. Heterozygous animals were crossed to an flp recombinase transgenic line for removal of the neo cassette. Subsequent mating to a cre recombinase transgenic line removed exon 1 and part of intron 1 leaving the GFP cassette intact. The exon 1 deletion removes the plasma membrane-targeting signal sequence and also brings the GFP cassette under the control of the endogenous promoter. RT-PCR analysis confirmed the removal of the signal sequence in gene transcripts from homozygous mutant animals. Immunohistochemistry of brain sections from homozygous mutants did not detect protein product. (J:76686)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Disease models
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Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Nrp2 Mutation:  30 strains or lines available
References
Original:  J:76686 Walz A, et al., Aberrant sensory innervation of the olfactory bulb in neuropilin-2 mutant mice. J Neurosci. 2002 May 15;22(10):4025-35
All:  8 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
02/25/2015
MGI 5.21
The Jackson Laboratory