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Targeted Allele Detail
Symbol: Eif2ak3tm1Drc
Name: eukaryotic translation initiation factor 2 alpha kinase 3; targeted mutation 1, Douglas R Cavener
MGI ID: MGI:2183829
Synonyms: Perk-
Gene: Eif2ak3  Location: Chr6:70844515-70905245 bp, + strand  Genetic Position: Chr6, 32.09 cM
Acinar cells are lost in both Eif2ak3tm1Drc/Eif2ak3tm1Drc and Eif2ak3tm1.1Drc/Eif2ak3tm1.1Drc Tg(Ela1-cre)16Eps/0 mice

Show the 1 phenotype image(s) involving this allele.

Germline Transmission:  Earliest citation of germline transmission: J:76661
Parent Cell Line:  TL1/TL-1 (ES Cell)
Strain of Origin:  129S6/SvEvTac
Allele Type:    Targeted (Null/knockout)
Mutation:    Intragenic deletion
Mutation detailsTwo loxP sites were introduced to intronic regions flanking 3 exons encoding part of the lumenal domain, the transmembrane domain, and part of the catalytic domain. A third loxP site was introduced with a PGK-neo cassette directly downstream of the second loxP site in the same intron. The 3 exons and PGK-neo cassette were excised by mating chimeric animals to a cre-expressing line. RT-PCR analysis of homozygous mutant animals using primers flanking the deleted region confirmed that gene transcripts did not contain the deleted exons. Western blot analysis using antibodies directed against the kinase domain did not detect protein product in pancreatic lysates from homozygous mutants. (J:76661)
View phenotypes for all genotypes (concatenated display).
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Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Eif2ak3 Mutation:  20 strains or lines available
Original:  J:76661 Zhang P, et al., The PERK eukaryotic initiation factor 2 alpha kinase is required for the development of the skeletal system, postnatal growth, and the function and viability of the pancreas. Mol Cell Biol. 2002 Jun;22(11):3864-74
All:  10 reference(s)

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MGI 6.03
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