Lat^tm2Lov
Targeted Allele Detail

Summary

• Symbol: Lat^tm2Lov
• Name: linker for activation of T cells; targeted mutation 2, Paul E Love
• MGI ID: MGI:2183740
• Synonyms: LAT^4YF-FLOX
• Gene: Lat Location: Chr7:125962996-125968742 bp, - strand Genetic Position: Chr7, 69.01 cM
• Alliance: Lat^tm2Lov page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77695
• Parent Cell Line: R1 (ES Cell)
• Strain of Origin: (129X1/SvJ x 129S1/Sv)F1-Kitl⁺

Mutation description

• Allele Type: Targeted
• Mutations: Insertion, Nucleotide substitutions
• Mutation details: Tyrosine to phenylalanine mutations were introduced at positions identified as being critical for the binding of signaling molecules (136, 175, 195, and 235) via site-directed mutagenesis. A floxed neomycin selection cassette was inserted downstream of the coding region. Mice carrying this allele were bred with transgenic mice expressing cre recombinase via the EIIa promoter to generate Lat^tm2.1Lov. (J:77695)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Lat Mutation: 11 strains or lines available

References

• Original: J:77695 Sommers CL, et al., Knock-in mutation of the distal four tyrosines of linker for activation of T cells blocks murine T cell development. J Exp Med. 2001 Jul 16;194(2):135-42
• All: 1 reference(s)