Map2k4^tm1Flv
Targeted Allele Detail

Summary

• Symbol: Map2k4^tm1Flv
• Name: mitogen-activated protein kinase kinase 4; targeted mutation 1, Richard A Flavell
• MGI ID: MGI:2183040
• Synonyms: MKK4 -
• Gene: Map2k4 Location: Chr11:65579069-65679123 bp, - strand Genetic Position: Chr11, 40.53 cM
• Alliance: Map2k4^tm1Flv page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:77628
• Parent Cell Line: W9.5/W95 (ES Cell)
• Strain of Origin: 129S1/Sv-Oca2⁺ Tyr⁺ Kitl⁺

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 3.5 kb fragment containg coding sequences for amino acids 211-268 was replaced with a neomycin selection cassette. These sequences include essential phosphorylation sites for protein activation. Western blot analysis on homozygous ES cells demonstrated that no detectable protein was made from this allele. (J:77628)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 0 strains available Cell Lines: 0 lines available
• Carrying any Map2k4 Mutation: 32 strains or lines available

References

• Original: J:77628 Yang D, et al., Targeted disruption of the MKK4 gene causes embryonic death, inhibition of c-Jun NH2-terminal kinase activation, and defects in AP-1 transcriptional activity. Proc Natl Acad Sci U S A. 1997 Apr 1;94(7):3004-9
• All: 5 reference(s)