Eif2ak2^tm1Cwe
Targeted Allele Detail

Summary

• Symbol: Eif2ak2^tm1Cwe
• Name: eukaryotic translation initiation factor 2-alpha kinase 2; targeted mutation 1, Charles Weissmann
• MGI ID: MGI:2182566
• Synonyms: N-PKR^-, PKR-, Pkr^o
• Gene: Eif2ak2 Location: Chr17:79159993-79190002 bp, - strand Genetic Position: Chr17, 49.56 cM, cytoband E2
• Alliance: Eif2ak2^tm1Cwe page

Mutation origin

• Germline Transmission: Earliest citation of germline transmission: J:30617
• Parent Cell Line: GS1 (ES Cell)
• Strain of Origin: 129/Sv

Mutation description

• Allele Type: Targeted (Null/knockout)
• Mutations: Insertion, Intragenic deletion
• Mutation details: A 2.0kb region, containing portions of exons 2 and 3, was replaced with a neomycin selection cassette adjoined to the 5' end of an upstream mouse sequence (UMS) element. Northern blot analyses, using various probes, showed that transcription was not terminated by the UMS element. RT-PCR and sequence analysis showed that exons 2 and 3 and the intervening neo cassette had been spliced out in the majority of transcripts. The exon-skipped transcript was shown to be capable of producing protein in a cell-free system, however, protein was undetected via Western blot analysis of lysates obtained from homozygous mutant mice. Additionally, assays of MEF cell extracts showed an absence of autophosphorylation activity in homozygous mutant mice. (J:30617)

Find Mice (IMSR)

• Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
• Carrying this Mutation: Mouse Strains: 2 strains available Cell Lines: 0 lines available
• Carrying any Eif2ak2 Mutation: 37 strains or lines available

References

• Original: J:30617 Yang YL, et al., Deficient signaling in mice devoid of double-stranded RNA-dependent protein kinase. EMBO J. 1995 Dec 15;14(24):6095-106
• All: 61 reference(s)