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Itgb8tm1Lfr
Targeted Allele Detail
Nomenclature
Symbol: Itgb8tm1Lfr
Name: integrin beta 8; targeted mutation 1, Louis F Reichardt
MGI ID: MGI:2182548
Gene: Itgb8  Location: Chr12:119158022-119238802 bp, - strand  Genetic Position: Chr12, 63.48 cM
Mutation
origin
Germline Transmission:  Earliest citation of germline transmission: J:77682
Parent Cell Line:  Other (see notes) (ES Cell)
Strain of Origin:  129/Sv
Mutation
description
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
 
Mutation detailsA PGK-neo-cassette replaced sequences encoding 45 amino acids at the C-terminus, corresponding to exon 4, which includes a von Willebreand factor-A-like domain, and part of the intron sequences. This targeting event resulted in a frameshift in addition to a deletion, abolishing functional Itgb8 protein. Northern blot and RT-PCR analysis verified the lack of functional transcript in homozygous mice. (J:77682)
Phenotypes
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View phenotypes for all genotypes (concatenated display).
Expression
In Mice Carrying this Mutation: 11 assay results
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 1 strain available      Cell Lines: 0 lines available
Carrying any Itgb8 Mutation:  18 strains or lines available
Notes
ES cell line = JMA7.
References
Original:  J:77682 Zhu J, et al., beta8 integrins are required for vascular morphogenesis in mouse embryos. Development. 2002 Jun;129(12):2891-903
All:  17 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Tumor Biology (MTB), Gene Ontology (GO), MouseCyc
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last database update
11/22/2016
MGI 6.06
The Jackson Laboratory