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Targeted Allele Detail
Symbol: Pck1tm1.1Lre
Name: phosphoenolpyruvate carboxykinase 1, cytosolic; targeted mutation 1.1, Lea Reshef
MGI ID: MGI:2182394
Synonyms: PPARE-
Gene: Pck1  Location: Chr2:173153073-173159274 bp, + strand  Genetic Position: Chr2, 95.79 cM, cytoband C1-qter
Germline Transmission:  Earliest citation of germline transmission: J:73977
Parent Cell Line:  E14 (ES Cell)
Strain of Origin:  129P2/OlaHsd
Allele Type:    Targeted (Null/knockout)
Mutations:    Insertion, Intragenic deletion
Mutation detailsThis allele is a derivative of Pck1tm1Lre in which Cre-mediated recombination removed the floxed PGK-neomycin resistance cassette that in the initial allele had replaced part of the adipose tissue-specific part of the promoter. Northern blot analysis of fasted homozygous mutant mice detected mRNA in liver and kidney, no mRNA in white adipose tissue, and a decreased level of mRNA in brown adipose tissue. RT-PCR studies using primers for exon 9 and 10 did not detect transcript in white adispose tissue from fasted homozygous mutant mice. (J:73977)
View phenotypes and curated references for all genotypes (concatenated display).
In Structures Affected by this Mutation: 5 anatomical structures
Find Mice (IMSR)
Mouse strains and cell lines available from the International Mouse Strain Resource (IMSR)
Carrying this Mutation:  Mouse Strains: 0 strains available      Cell Lines: 0 lines available
Carrying any Pck1 Mutation:  6 strains or lines available
Original:  J:73977 Olswang Y, et al., A mutation in the peroxisome proliferator-activated receptor gamma-binding site in the gene for the cytosolic form of phosphoenolpyruvate carboxykinase reduces adipose tissue size and fat content in mice. Proc Natl Acad Sci U S A. 2002 Jan 22;99(2):625-30
All:  4 reference(s)

Contributing Projects:
Mouse Genome Database (MGD), Gene Expression Database (GXD), Mouse Models of Human Cancer database (MMHCdb) (formerly Mouse Tumor Biology (MTB), Gene Ontology (GO)
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MGI 6.14
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